- ￥4800
- Tanda
- 美国
- TD-26163
- 2025年10月31日
- ELISA, WB, IHC
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin.
- 亚型：
lgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26163
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IHC
- 浓度：
1mg/ml
- 靶点：
R172K
- 抗体英文名：
Anti IDH2(R172K) Mouse Monoclonal Antibody
- 抗体名：
IDH2(R172K)
- 规格：
100 μL
IDH2(R172K)
| Cat. #: TD-26163 |
| Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
| Description: Anti-IDH2(R172K) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas. |
| Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 IHC: 1:10-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: recognizes R172K mutant, but not wild type IDH2 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant IDH2(R172K) and wild type proteins.
Purified His-tagged IDH2(R172K) (lane 2) and wild type protein (lane 1) were blotted with Anti-IDH2(R172K) monoclonal antibody (Cat. #TD-26163).
Immunofluorescence:
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172K) antibody.
HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid or pCDNA3-GFP- IDH2 (R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (Cat. #TD-26163).
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文献和实验【原创】NCBI中查询出的cds序列中出现了k,r,m,请问代表什么意思呢？
ttcgataccc ctgacatgat caacaccaag gttg // } 奇怪的是序列中出现了r,m,k，请问是什么意思呢？ 在NCBI的链接如下： [url][/url]http://www.ncbi.nlm.nih.gov/nuccore/52630747?from=1&to=704&report=gbwithparts
2 Hybrid System TRAFO Protocol
glycol (LiAc/ss-DNA/PEG) protocol. Technical Tips Online ( http://tto.trends.com ). For complete instructions on how to do a two hybrid screen see the following references. 1. Gietz, R.D., B. Triggs-Raine, A. Robbins, K.C. Graham, and R
〕 / 〔 Rn〕大于 1。但是如果底物仅和 R结合，对这种蛋白质溶液加入底物，随其浓度逐渐上升，脱离 Rn状态的分子的比率逐渐增加。现在废物 -酶复合物的解离常数是 Ks，底物浓度为〔 S〕，若令〔 S〕 /Ks=a，在 越大， S型的特点就越显著。因此可以认为负效是使 L变大，正效是使 L变小。与此相对应的 K-N-F理论指出，在底物不存在的场合，原基均处于 Tn状态，与底物结合时，带有底物的原基仅处于 R状态。因此若结合 m个底物，就成为 Tn-mRm的状态。从 Tn-mRm〔 s
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