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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant p53(R282W) protein, human origin.
- 亚型:
IgG1
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
单克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白
- 目录编号:
TD-26076
- 级别:
科研级
- 库存:
999
- 供应商:
武汉天德
- 宿主:
Mouse
- 应用范围:
ELISA, WB, IHC, IP
- 浓度:
1mg/ml
- 靶点:
R282W
- 抗体英文名:
Anti TP53(R282W) Mouse Monoclonal Antibody
- 抗体名:
TP53(R282W)
- 规格:
100 μL
TP53(R282W)
| Cat. #: TD-26076 |
| Gene Symbol: TP53 |
| Description: Anti-TP53(R282W) Mouse Monoclonal Antibody |
| Background: p53 protein acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are found in many types of cancers. |
| Immunogen: Recombinant p53(R282W) protein, human origin. |
| Applications: ELISA, WB, IHC, IP |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG1 |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-TP53(R282W) antibody recognizes TP53(R282W) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Immunofluorescence:
|
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文献和实验Science:直击肿瘤命门,Shibin Zhou 等开发靶向 TP53 突变的双特异性抗体
Q, R248W, R249S, R273C, R273H,R273L, R282W 进行了检测和验证。研究人员又对 28 例患者中分离出的 TILs 进行分析发现,有 11 例具有靶向 TP53 常见突变的 T 细胞反应,其中鉴定出的唯一共有的突变的 TP53 新表位是 HLA*02:01 R175H(HMTEVVRHC)。 图片来源:The Journal of clinical investigation 也就是说, HLA*02:01 R175H(HMTEVVRHC)这种新抗原突变并不
;(B)绿色荧光蛋白(GFP)标记的TYK-nu与T细胞共培养,E:T比为5:1,有或没有H2-scDb在Incucyte®系统中连续监测,显示24小时和96小时拍摄的相位和绿色荧光图像。 研究人员基于 CRISPR 的技术对携带内源性 HLA-A*02:01 和 p53R175H 的 KMS26、KLE 和 TYK-nu 癌细胞系中的 TP53 进行了基因破坏。H2-scDb 介导的细胞毒性通过破坏这些细胞中的 TP53 类似地减轻,通过Incucyte® 连续检测超过120h,呈现动态的定量
TP53 Mutation Detection by SSCP and Sequencing
The TP53 tumor suppressor gene on chromosome 17p13.1 contains 11 exons and encodes a nuclear phosphoprotein of 53 kDa, a transcription factor involved in the regulation of the cell cycle (1 ). Normal p53 protein functions as a cell cycle
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