- ￥4800
- Tanda
- 美国
- TD-26418
- 2025年10月27日
- ELISA, WB, IHC
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of SMAD4 which includes the mutation of D351H, huma n origin.
- 亚型：
lgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26418
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IHC
- 浓度：
1mg/ml
- 靶点：
D351H
- 抗体英文名：
Anti-SMAD4 (D351H) Mouse Monoclonal Antibody
- 抗体名：
SMAD4 (D351H)
- 规格：
100 μL
SMAD4 (D351H)
Catalog Number: TD-26418
Gene Symbol: SMAD4, DPC4, JIP, MADH4, MYHRS
Description: Anti-SMAD4 (D351H) Mouse Monoclonal Antibody
Background: SMAD4 family member 4, also known as SMAD4, is a protein that in huma n is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-β signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma . It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominantdisease juvenile polyposis syndrome (JPS).
Immunogen: A synthetic peptide from the internal region of SMAD4 which includes the mutation of D351H, huma n origin.
Tested applications: ELISA, WB, IHC
Recommended dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration: 1 mg/ml
Host: Mouse
Clonality: Monoclonal
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes D351H mutant, but not wild-type SMAD4 of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.
Western blot:
Western blot analysis of recombinant SMAD4 (D351H) and wildtype proteins. Purified His-tagged SMAD4 (D351H) protein (lane1) and corresponding wild-type protein (1-552aa, 65kd) (lane2) were blotted with anti-SMAD4 (D351H) monoclonal antibody (Cat. #TD-26418).
mmunofluorescence:
Immunofluorescence of cells expressing SMAD4 proteins with anti-SMAD4 (D351H) antibody. HEK293T cells were transfected with pCDNA3-GFP-SMAD4 (D351H) plasmid ,pCDNA3-GFP-SMAD4 (WT) plasmid or pCDNA3-GFP-SMAD4 (D351N) plasmid, then fixed and stained with anti-SMAD4 (D351H) monoclonal antibody (Cat. #TD-26418).
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文献和实验dingding2004 想找一个药物来阻断SMAD4 的作用。不知有没有做过的。 SMAD4 是在转录水平发挥作用的，如果有这种药物，加在要干预的细胞培养液中，可以发挥作用吗？ dingding2004 自己顶下啊 caoying207 唉，没想到2年以后，我也在找SMAD4阻断剂（抑制剂 ），前辈，你找到了吗？ skyzhuz 半
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CONVENTIONAL TWO STEP FIXATION [See D.H. Hall, 1995]
"CONVENTIONAL" TWO STEP FIXATION [See D.H. Hall, 1995] 1. Wash animals in M9 and anaesthetized in 8% alcohol in M9, 5’ 2. 2.5% glutaraldehyde, 1% paraformaldehyde in 0.1M sucrose, 0.05M cacodylate on ice, 2 hours 3. 3 rinses in 0.2
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