Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of (L747-P753) S, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes EGFR(L747-P753)S, but not wild type EGFR protein from vertebrates.
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing
Western blot:
Western blot analysis of recombinant EGFR(L747-P753) S and wild type proteins.
Purified His-tagged EGFR(L747-P753)S protein (702-987) and wild type protein (702-987) were blotted with anti- EGFR(L747-P753)S mouse antibody (Cat. #TD-26287).
Immunofluorescence:
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(L747-P753)S antibody.
HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(L747-P753)S plasmid (right column), then fixed and stained with anti- EGFR(L747-P753)S monoclonal antibody (Cat. #TD-26287).