- ￥4800
- Tanda
- 美国
- TD-26287
- 2025年10月25日
- ELISA, WB, IF, IHC
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of EGFR which includes the mutation of (L747-P753) S, human origin.
- 亚型：
lgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26287
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IF, IHC
- 浓度：
1mg/ml
- 靶点：
L747-P753
- 抗体英文名：
Anti EGFR(L747-P753)S Mouse Monoclonal Antibody
- 抗体名：
EGFR (L747-P753)S
- 规格：
100 μL
EGFR (L747-P753)S
| Cat. #: TD-26287 |
| Gene Symbol: EGFR/HER1/ERBB1 |
| Description: Anti-EGFR(L747-P753)S Mouse Monoclonal Antibody |
| Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). |
| Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of (L747-P753) S, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes EGFR(L747-P753)S, but not wild type EGFR protein from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant EGFR(L747-P753) S and wild type proteins.
Purified His-tagged EGFR(L747-P753)S protein (702-987) and wild type protein (702-987) were blotted with anti- EGFR(L747-P753)S mouse antibody (Cat. #TD-26287).
Immunofluorescence:
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(L747-P753)S antibody.
HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(L747-P753)S plasmid (right column), then fixed and stained with anti- EGFR(L747-P753)S monoclonal antibody (Cat. #TD-26287).
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文献和实验Phosphoamino acid analysis:Mark Kamps's method
Recipes for the buffers are at the end of this protocol. 1.Label your protein with 32Pi.Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P. Neither nitrocellulose nor nylon
死亡。显示了其治疗价值。2、抗缺失突变性EGFR单克隆抗体及多肽（1） PEPHC1PEPHC1是人工合成多肽[12] 。它可与突变型和野生型EGFR胞外区结合。抑制EGFR与配体结合，阻断信号传导通路。（2） MAB806MAB806与突变型EGFR有较高亲和力（1*109m-1）[13]，且特异性结合于缺失突变的基因融合区。实验证实了MAB806与高表达突变型EGFR的肿瘤细胞结合，可克服对正常组织的损坏。（3）L8A4和3C10L8A4和3C10是针对缺失突变型EGFR胞外区连接处多肽的单克
Clinical tools to accurately describe, evaluate, and predict an individual’s response to cancer therapy are a field-wide priority; in many advanced cancers, only 10–20% of individuals will have a clinical benefit from therapy, yet we treat
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