Catalog Number: TD-26352 Gene Symbol: CASP8; ALPS2B; CAP4; MACH; FLICE Description: Anti-CASP8 Mouse Monoclonal Antibody Background: The CASP8 gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. A very rare genetic disorder of the immune system can also be caused by mutations in this gene. Immunogen: Purified CASP8 protein, human origin. Tested applications: ELISA, WB, IHC Recommended dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 Concentration: 1 mg/ml Host: Mouse Clonality: Monoclonal Purity: Purified from ascites Format: Liquid Storage buffer: Preservative: no Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity: Recognizes CASP8 of vertebrates. Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. Western blot: Western blot analysis of recombinant CASP8 proteins. Purified His-tagged CASP8 protein (268-435aa, 25KD) was blotted with anti-CASP8 monoclonal antibody (Cat. #TD-26352). Immunofluorescence:
Immunofluorescence of cells expressing CASP8 proteins with anti-CASP8 antibody. HEK293T cells were transfected with pCDNA3-GFP-CASP8 (WT) plasmid, then fixed and stained with anti-CASP8 monoclonal antibody (Cat. #TD-26352).