- ￥4800
- Tanda
- 美国
- TD-26039
- 2025年10月29日
- ELISA, WB, IHC, IP
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of BRaf(V600E), human origin.
- 亚型：
IgG2b
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26039
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IHC, IP
- 浓度：
0.5 mg/ml
- 靶点：
V600E
- 抗体英文名：
Anti BRaf(V600E) Mouse Monoclonal Antibody
- 抗体名：
B-Raf (V600E)
- 规格：
100 μL
B - Raf (V600E)
Catalog Number: TD-26039
Gene Symbol: B - Raf (V600E)
Description: Anti- B - Raf (V600E) Mouse Monoclonal Antibody
Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome . Acquired mutations in BRAF have been found in cancers
Immunogen: A synthetic peptide from the internal region of BRaf(V600E)， human origin.
Tested applications: ELISA, WB, IHC , IP
Tested applications: ELISA, WB, IHC, IP
Recommended dilutions:
ELISA 1:100-1:3000
WB 1:200-1:1000
IHC 1:10-1:1000
Concentration: 0.5 mg/ml
Host: mouse
Clonality: Monoclonal
Isotype: IgG2b
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes B-Raf(V600E), but not wild type B-Raf proteins from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of recombinant B-raf proteins with anti-BRaf(V600E) antibody.
Purified His - tagged B-Raf( V600E )（amino acids 513-693)(lane1) ， corresponding wild type protein(lane 2),His - tagged B-Raf( V600D ) (lane 3) ,
His - tagged B-Raf( V600A ) (lane 4) ,His - tagged B-Raf( V600G ) (lane 5) ,His - tagged B-Raf( V600K ) (lane 6),His - tagged B-Raf( V600R ) (lane 7), and His - tagged B-Raf( V600M ) (lane 8) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat # TD-26039) .
Immunofluorescence:
Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP- B-Raf( V600E )plasmid,pCDNA3-GFP- B-Raf( WT )plasmid,pCDNA3-GFP- B-Raf( V600K )plasmid,pCDNA3-GFP- B-Raf( V600R )plasmid,pCDNA3-GFP- B-Raf( V600A )plasmid,pCDNA3-GFP- B-Raf( V600D ) plasmid,pCDNA3-GFP-B-Raf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal antibody (Cat. # TD-26039).
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文献和实验Assaying for BRAF V600E in Tissue and Blood in Melanoma
The BrafV600E mutation has been detected in patients with metastatic melanoma, colon, thyroid, and other cancers. Studies suggested that tumors with this mutation are especially sensitive to BRAF inhibitors-hence the need to reliably
Monitoring Oncogenic B-RAF-Induced Senescence in Melanocytes
The B-RAF kinase is a downstream effector of the RAS family of proto-oncogenes and is constitutively activated in the majority of human melanomas. The common oncogenic B-RAFV600E mutant cooperates with additional genetic lesions to transform
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