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- Fluoroprobes
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- 2026年01月03日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20
- 英文名：
Sulfo-Cy5 NHS ester
- 库存：
1
- 供应商：
靶点科技
- 规格：
5 mg
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- 询问日期
文献和实验were prepared by labeling BSA with Cy3 and Cy5 monofunctional reactive dyes (Amersham Pharmacia Biotech). Cy5-BSA-biotin was prepared by labeling Cy5-BSA with Sulfo-NHS-LC-Biotin (Pierce). Cy3-BSA-maleimide was prepared by labeling Cy3-BSA with Sulfo-GMBS
Revised amino-allyl labeling protocol
C indefinitely. D. Coupling Add 0.5 m L 1M sodium bicarbonate, pH 9.0 to 50 mM final. Check 1M stock solution periodically for fluctuations in pH. Monofunctional NHS-ester Cy3 (PA23001) and Cy5 dye (PA25001, Amersham) is supplied
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
in pH. Monofunctional NHS-ester Cy3 (PA23001) and Cy5 dye (PA25001, Amersham) is supplied as a dry pellet. Each tube is sufficient to label 10 reactions under normal conditions. Dissolve dry pellet in 20 mL DMSO. Aliquot 2 mL into 10 single
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