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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
MB 660 Azide
- 供应商：
靶点科技
- 规格：
1 mg
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文献和实验Replication timing by comparative hybridization
cells per time point. (Raffinose has not worked well for me, so I avoid it.) 2. Arrest the culture with alpha factor (200 nM final concentration for bar1 strains) when the OD660 is about 0.2-0.25. 3. When the cells have arrested (more than 95
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
Replication timing using transient hemimethylation
, and the transformant exhibiting the highest level of DpnI cleavage was selected (RM14-3a Meth5 strain). Protocol for methylation timing 1. Grow ~330 ml of RM14-3a expressing dam methylase (RM14-3a Meth5) to an OD660 of about 0.3 (3.85 x 106 cells/ml) at 23o C
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