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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
AFDye 594 Azide
- 供应商:
靶点科技
- 规格:
1 mg
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文献和实验v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
for upto 1 month (add 0.02% [w/v] sodium azide to prevent bacterialgrowth). Dilute the appropriate secondary antibody ina solution containing 1% (w/v) milk in TBS-T according tothe manufacturer’s instructions (usually 1:2,500–1:10,000). Incubate
Evaluation of Proliferation of Neural Stem Cells In Vitro and In Vivo
15‐ml and 50‐ml conical centrifuge tubes (sterile; e.g., BD Falcon) Stereomicroscope (e.g., Zeiss Stemi DV4 Stereo Microscope, cat. no. NT55‐594
4% paraformaldehyde (PFA; see recipe ) 0.1 M PB (see recipe ) with and without 0.05% sodium azide, 4°C
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