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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
Methylamp One-Step DNA Modification Kit (40 samples)
- 规格:
40次样本
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文献和实验长度可达 20kb,适合于各种 GC 含量的扩增,可用于多种样本的直接 PCR 如植物叶片、全血等。且扩增仅需 30s/kb,甚至于 2kb/s。如此卓越,爱不释手了吧? 4. 选择直接扩增试剂盒对粗品扩增 vazyme 生产的各种直扩试剂盒,直接对粗品进行扩增,大大减少了实验的工作周期。如小鼠基因型鉴定可以直接使用 One Step Mouse Genotyping Kit,全血扩增可以直接使用 Blood Direct PCR Kit V2。
after PCR amplification with or without cloning. This protocol also describes procedure for handling nanogram quantities of DNA . Materials and Regents Prepare immediately before use 10 mM hydroquinone (Sigma; #H 9003) 40.5% sodium bisulfite (Sigma
Bisulfite modification kits and PCR-DNA Methylation, His
, the chances of getting 400 bp amplification are high and the modified DNA should be also OK for MSP. For modification, use 1-2 μg DNA and elute in 30 μl water at the last step. Use 2 μl for a 20 μl PCR reaction. If you are amplifying DNA for sequencing
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