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- 文献和实验
- 技术资料
- 英文名:
PHF2 Monoclonal Antibody [3E7] (200 ul)
- 供应商:
上海西宝
- 规格:
200 ul
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文献和实验Monoclonal Antibody Production Protocol
/ml). Add Aminopterin (2ml to 200 ml of medium). (Some workers will leave the cells at this point for 24 hours before adding Aminopterin. We add the drug immediately.) Seed the cells in 96 well microtiter dishes, 250 ul per well, 8 plates per fusion
Polyclonal and Monoclonal Antibodies
Research, Inc. ), the latter being less hazardous than the first. 1) 1st injection: 15 - 50 ug Antigen in 200 ul adjuvant, injected subcutaneously. 2) 2nd injection: 2 weeks later; boost with 15 - 50 ug of Antigen in adjuvant. (If time permits
The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
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