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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
西宝生物
- 英文名:
TRIS-GLYCINE BUFFER
- 保存条件:
Invalid
- 库存:
1 box
- 规格:
BIOCHEMICALS
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文献和实验How to make DEPC-treated water and Tris Buffer
first, and then dissolve Tris to make the appropriate buffer. Trace amounts of DEPC will modify purine residues in RNA by carboxymethylation. Carboxymethylated RNA is translated with very low efficiency in cell-free systems. However, its ability to form DNA:RNA or RNA:RNA
from the bottom of the cassette. 3) In one smooth motion, gently pull the comb out of the cassette. 4) Rinse the sample wells with the appropriate 1X SDS Running Buffer. Invert the gel and shake the gel to remove the buffer. Repeat two more times.
【求助】shRNA连接时,如果没有annealing buffer,可不可以用PCR buffer?
bobbymm shRNA要做连接的时候发现 annealing buffer 没有了。可以用 PCR buffer代替不? zhujoker 就用普通的去离子水就可以了,没有那么复杂。不要想当然得使用PCR buffer。 bobbymm 用的是TAKARA的PCR BUFFER ,连接的还不错。 已经送去测序了。 谢谢回复。 本文
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