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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
广东固康生物科技有限公司
- 检测范围：
——
- 检测方法：
BCA方法学
- 应用：
——
- 适应物种：
人
- 标记物：
——
- 样本：
血清/血浆
- 规格：
套
【产品名称】
通用名称：阿狄科®蛋白质定量检测（BCA方法学）
英文名称：Protein quantification test (BCA method)
【预期用途】
用于测定人血清和血浆中蛋白质。仅供科研使用。
【背景知识】
活性氧 (ROS) 可以氧化细胞中的蛋白质、脂质和 DNA，从而在结构和功能上破坏它们。蛋白质被自由基氧化，以各种方式修饰或降解其氨基酸。蛋白质接受新的官能团，如羰基或羟基，可导致蛋白质断裂、交联形成、三级结构破坏和功能丧失。活性氧还与动脉粥样硬化、阿尔茨海默氏症、帕金森氏症和类风湿性关节炎等疾病直接相关以及衰老过程和致癌作用。
蛋白质结合羰基化合物由多种氧化机制形成，是氧化损伤的敏感标志物。蛋白质结合羰基化合物的含量可以通过用DNPH衍生化并随后测定结合的抗 DNPH 抗体来确定。 ELISA 方法能够以微克范围内的蛋白质含量进行定量羰基测定。
适应症：
通用名称：阿狄科®蛋白质定量检测（BCA方法学）
英文名称：Protein quantification test (BCA method)
【预期用途】
用于测定人血清和血浆中蛋白质。仅供科研使用。
【背景知识】
活性氧 (ROS) 可以氧化细胞中的蛋白质、脂质和 DNA，从而在结构和功能上破坏它们。蛋白质被自由基氧化，以各种方式修饰或降解其氨基酸。蛋白质接受新的官能团，如羰基或羟基，可导致蛋白质断裂、交联形成、三级结构破坏和功能丧失。活性氧还与动脉粥样硬化、阿尔茨海默氏症、帕金森氏症和类风湿性关节炎等疾病直接相关以及衰老过程和致癌作用。
蛋白质结合羰基化合物由多种氧化机制形成，是氧化损伤的敏感标志物。蛋白质结合羰基化合物的含量可以通过用DNPH衍生化并随后测定结合的抗 DNPH 抗体来确定。 ELISA 方法能够以微克范围内的蛋白质含量进行定量羰基测定。
适应症：
- 动脉硬化
- 阿尔茨海默氏症
- 帕金森氏症
- 类风湿关节炎
- 尿毒症
- 糖尿病
- 衰老过程
- 致癌
参考文献：
1. Beal, M. F. Oxidatively modified proteins in aging and disease.Free radical biology& medicine 32,797-803(2002).
2. Berlett,B. S.& Stadtman, E.R.Protein oxidation in aging, disease, and oxidative stress.The Journal of biological chemistry 272,20313-6 (1997).
3. Buss, H., Chan,T. P., Sluis,K. B., Domigan,N. M.& Winterbourn, C.C. Protein carbo-nyl measurement by a sensitive ELISA method.Free radical biology & medicine 23,361-6 (1997).
4. Cao, G.& Cutler,R.G.Protein oxidation and aging.I. Difficulties in measuring re-active protein carbonyls in tissues using 2,4-dinitrophenylhydrazine.Archives ofbiochemistry and biophysics 320,106-14(1995).
5. Davies, K. J.,Delsignore,M.E.& Lin, S. W. Protein damage and degradation by oxygen radicals.ll.Modification of amino acids. TheJournal of biological chemistry262,9902-7(1987).
6. Dean,R.T., Fu, S., Stocker,R.& Davies,M.J.Biochemistry and pathology of radical-mediated protein oxidation.The Biochemical journal324 ( Pt 1,1-18(1997).
7. Descamps-Latscha，B.& Witko-Sarsat,V. lmportance of oxidatively modified proteins in chronic renal failure.Kidney international. Supplement 78,S108-13(2001).
8. Galli, F. Protein damage and inflammation in uraemia and dialysis patients. Ne-phrology, dialysis, transplantation : official publication of the European Dialysis andTransplant Association-European Renal Association 22 Suppl 5, v20-36 (2007).
9. Gladstone,L. M.& Levine, R.L. Oxidation of proteins in neonatal lungs. Pediatrics93,764-8 (1994).
10.Lenz, a G.et al. Oxidatively modified proteins in bronchoalveolar lavage fluid of patients with ARDS and patients at-risk for ARDS.The European respiratory journal13,169-74 (1999).
11.Levine,R.L.Carbonyl modified proteins in cellular regulation, aging, and disease.Free radical biology & medicine 32,790-6(2002).
12.Levine,R.L.& Stadtman,E.R. Oxidative modification of proteins during aging.Experimental gerontology 36,1495-502 (2001).
13.Marnett,L. J.， Riggins，J. N. & West,J. D. Endogenous generation of reactive oxidants and electrophiles and their reactions with DNA and protein.The Journalof clinical investigation 111,583-93(2003).
14.Matzi, V.et al.The impact of preoperative micronutrient supplementation in lung surgery.A prospective randomized trial of oral supplementation of combinedalpha-ketoglutaric acid and 5-hydroxymethylfurfural.European journal of cardio-thoracic surgery : officialjournal of the European Association for Cardio-thoracic Sur-gery 32,776-82(2007).
15.Reznick，A. Z. et al. Modification of plasma proteins by cigarette smoke as measured by protein carbonyl formation.The Biochemical journal286(Pt 2,607-11(1992).
16.Smith, C. D. et al.Excess brain protein oxidation and enzyme dysfunction in nor-mal aging and in Alzheimer disease. Proceedings of the National Academy of Sci-ences of the United States of America 88,10540-3 (1991).
17.Stadtman,E.R.& Oliver,C.N. Metal-catalyzed oxidation of proteins. Physiological consequences.The Journal of biological chemistry 266,2005-8 (1991).
18.Starke-Reed, P. E.& Oliver,C. N. Protein oxidation and proteolysis during aging and oxidative stress.Archives of biochemistry and biophysics 275,559-67 (1989).
19. Wiseman,H.& Halliwell,B. Damage to DNA by reactive oxygen and nitrogen spe-cies: role in inflammatory disease and progression to cancer. The Biochemicaljour-nal 313 ( Pt 1,17-29 (1996).
1. Beal, M. F. Oxidatively modified proteins in aging and disease.Free radical biology& medicine 32,797-803(2002).
2. Berlett,B. S.& Stadtman, E.R.Protein oxidation in aging, disease, and oxidative stress.The Journal of biological chemistry 272,20313-6 (1997).
3. Buss, H., Chan,T. P., Sluis,K. B., Domigan,N. M.& Winterbourn, C.C. Protein carbo-nyl measurement by a sensitive ELISA method.Free radical biology & medicine 23,361-6 (1997).
4. Cao, G.& Cutler,R.G.Protein oxidation and aging.I. Difficulties in measuring re-active protein carbonyls in tissues using 2,4-dinitrophenylhydrazine.Archives ofbiochemistry and biophysics 320,106-14(1995).
5. Davies, K. J.,Delsignore,M.E.& Lin, S. W. Protein damage and degradation by oxygen radicals.ll.Modification of amino acids. TheJournal of biological chemistry262,9902-7(1987).
6. Dean,R.T., Fu, S., Stocker,R.& Davies,M.J.Biochemistry and pathology of radical-mediated protein oxidation.The Biochemical journal324 ( Pt 1,1-18(1997).
7. Descamps-Latscha，B.& Witko-Sarsat,V. lmportance of oxidatively modified proteins in chronic renal failure.Kidney international. Supplement 78,S108-13(2001).
8. Galli, F. Protein damage and inflammation in uraemia and dialysis patients. Ne-phrology, dialysis, transplantation : official publication of the European Dialysis andTransplant Association-European Renal Association 22 Suppl 5, v20-36 (2007).
9. Gladstone,L. M.& Levine, R.L. Oxidation of proteins in neonatal lungs. Pediatrics93,764-8 (1994).
10.Lenz, a G.et al. Oxidatively modified proteins in bronchoalveolar lavage fluid of patients with ARDS and patients at-risk for ARDS.The European respiratory journal13,169-74 (1999).
11.Levine,R.L.Carbonyl modified proteins in cellular regulation, aging, and disease.Free radical biology & medicine 32,790-6(2002).
12.Levine,R.L.& Stadtman,E.R. Oxidative modification of proteins during aging.Experimental gerontology 36,1495-502 (2001).
13.Marnett,L. J.， Riggins，J. N. & West,J. D. Endogenous generation of reactive oxidants and electrophiles and their reactions with DNA and protein.The Journalof clinical investigation 111,583-93(2003).
14.Matzi, V.et al.The impact of preoperative micronutrient supplementation in lung surgery.A prospective randomized trial of oral supplementation of combinedalpha-ketoglutaric acid and 5-hydroxymethylfurfural.European journal of cardio-thoracic surgery : officialjournal of the European Association for Cardio-thoracic Sur-gery 32,776-82(2007).
15.Reznick，A. Z. et al. Modification of plasma proteins by cigarette smoke as measured by protein carbonyl formation.The Biochemical journal286(Pt 2,607-11(1992).
16.Smith, C. D. et al.Excess brain protein oxidation and enzyme dysfunction in nor-mal aging and in Alzheimer disease. Proceedings of the National Academy of Sci-ences of the United States of America 88,10540-3 (1991).
17.Stadtman,E.R.& Oliver,C.N. Metal-catalyzed oxidation of proteins. Physiological consequences.The Journal of biological chemistry 266,2005-8 (1991).
18.Starke-Reed, P. E.& Oliver,C. N. Protein oxidation and proteolysis during aging and oxidative stress.Archives of biochemistry and biophysics 275,559-67 (1989).
19. Wiseman,H.& Halliwell,B. Damage to DNA by reactive oxygen and nitrogen spe-cies: role in inflammatory disease and progression to cancer. The Biochemicaljour-nal 313 ( Pt 1,17-29 (1996).
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文献和实验相关实验
主要特点：· 准确灵敏：BCA试剂的蛋白质测定范围是20－2000μg/ml,采用加强方法可检测到5μg/ml；MicroBCA试剂测定范围是0.5-20μg/ml。· 快速：45分钟内完成测定，比经典的Lowry法快4倍而且更加方便。· 经济实用：除试管外，测定可在微孔板中进行，大大节约样品和试剂用量。· 不受样品中离子型和非离子型去污剂影响。· 检测不同蛋白质分子的变异系数远小于考玛斯亮蓝。 基本原理： 碱性条件下，蛋白将Cu ++ 还原
简述：传统标准的BCA蛋白定量分析方法常规均使用试管或者微孔板进行检测，通过对传统方法进行改进，可使用BioTek公司的Take3TM 超微量多体积检测板进行原位微量BCA法蛋白定量分析。待测蛋白样品和BCA工作缓冲液按照顺序直接加在Take3TM板的微量定量孔处、温浴，使用EpochTM 微孔板分光光度计进行检测。和传统标准BCA方法（BCA工作缓冲液和蛋白质样品体积比为20:1）相比，该方法的蛋白检测灵敏度有明显的提高。相比与Mini-BCA分析方法，原位分析方法更加精确。介绍BCA
® TQ-S MS可实现与免疫检测方法相匹配的高灵敏度，但与后者相比更加省时省力 ■ 与传统LBA方法相比更为准确精密 ■ 分析速度快，只需3.5分钟，大大提高了工作效率 详细内容：人血浆中缓激肽的SPE LC-MS/MS定量检测方法开发
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阿狄科®蛋白质定量检测（BCA方法学）
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