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参照说明书
- 英文名：
Signal-Seeker™ Ubiquitin Enrichment Kit (30 assays)
- 库存：
1
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
KIT30assays
The Signal-Seeker™ line of produts have been developed to allow simple analysis of key regulatory protein modifications by specialists and non-specialists alike. The comprehensive Signal-Seeker™ kits provide an affinity bead system to isolate and enrich modified proteins from any given cell or tissue lysate. The enriched protein population is then analyzed by standard western blot procedures using a primary antibody to the target protein.
Product Uses Include
- Investigate transient regulatory mechanisms
- Measure signalling events of multiple pathway member proteins
- Discover new modifications of your protein of interest
- Gain insight into regulatory mechanisms
- Measure endogenous or transiently expressed protein signalling events
-
Kit contents
The Ubiquitination kit contains the following components:
Lysis and protein quantitation step IP and pre-clear step Wash step Elution step Western step BlastR™ Lysis Buffer
BlastR™ Dilution Buffer
BlastR™ Filters
Protease Inhibitor Cocktail
De-Ubiquitinase inhibitor Cocktail
Precision Red™ Protein Assay Reagent
Ubiquitination Affinity Beads
Ubiquitination Control Beads
BlastR™ Wash Buffer
Spin Columns
Bead Elution Buffer
Chemiluminescent Reagent A
Chemiluminescent Reagent B
Anti-ubiquitin-HRP antibody
Example results
There are many applications for these kits (see next section below), here we describe an interesting application for Rac1 protein:
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文献和实验Studies of the Ubiquitin Proteasome System
ubiquitin buffer 2 for E3 assays (see recipe ) 10 mg/ml ubiquitin 4× reducing sample buffer 30°C Thermomixer (Eppendorf), or other heated shaker Boiling water bath
(2-10 μg): RiboMinus™ Probe (100 pmol/μl): 4 μl Hybridization Buffer (B10): 100 μl 2) Incubate the tube at 37°C for 5 minutes to denature RNA. 3) Place the sample on ice for at least 30 seconds 4. Proceed
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
Brief protocol using Digest-All™ Kit is described below. The Digest-All™ is a flexible and standardized system for tissue digestion and contains trypsin, ficin, and pepsin proteolytic enzymes. The degree of digestion is based on a standardized 10 minute
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