Cytoskeleton公司成立于1993年,专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、蛋白质转录后修饰(PTM)、细胞骨架研究相关的系列试剂盒和产品,尤其以细胞骨架相关研究见长,既能满足于样品较少的科学研究,也可以用于小规模筛选研究和高通量大规模筛选研究。此外,公司还提供微管蛋白,肌动蛋白,小G蛋白,GAPs,GEFs等现有产品的药物筛选服务。
The Signal-Seeker™ line of produts have been developed to allow simple analysis of key regulatory protein modifications by specialists and non-specialists alike. The comprehensive Signal-Seeker™ kits provide an affinity bead system to isolate and enrich modified proteins from any given cell or tissue lysate. The enriched protein population is then analyzed by standard western blot procedures using a primary antibody to the target protein.
Product Uses Include
Investigate transient regulatory mechanisms
Measure signalling events of multiple pathway member proteins
Discover new modifications of your protein of interest
Gain insight into regulatory mechanisms
Measure endogenous or transiently expressed protein signalling events
Kit contents
The Ubiquitination kit contains the following components:
Lysis and protein quantitation step
IP and pre-clear step
Wash step
Elution step
Western step
BlastR™ Lysis Buffer
BlastR™ Dilution Buffer
BlastR™ Filters
Protease Inhibitor Cocktail
De-Ubiquitinase inhibitor Cocktail
Precision Red™ Protein Assay Reagent
Ubiquitination Affinity Beads
Ubiquitination Control Beads
BlastR™ Wash Buffer
Spin Columns
Bead Elution Buffer
Chemiluminescent Reagent A
Chemiluminescent Reagent B
Anti-ubiquitin-HRP antibody
Example results
There are many applications for these kits (see next section below), here we describe an interesting application for Rac1 protein: