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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Pools of purified homogenous IgG2a isolated from pooled mouse sera belonging to the allelic types Igh- 1a and Igh-1b. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 形态:
Lyophilized from PBS pH 7.2
- 保存条件:
The lyophilized conjugate is shipped at ambient te
- 克隆性:
Polyclonal
- 适应物种:
Mouse IgG2a
- 级别:
分子生物学级
- 供应商:
Agrisera
- 宿主:
Goat
- 应用范围:
1 : 1000-1 : 10 000 (ELISA), 1 : 100-1 : 500 (IHC)
- 靶点:
The reactivity of the antiserum is directed to the subclass IgG2a. It reacts with IgG2a of the allelic types Igh-1a and Igh-1b, which include BALB/C, C57Bl, CBA/J, SJL/J and SM/J. hen used for the identification of IgG2a in other mouse strains the reactio
- 抗体英文名:
This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. However this conjugate has been passed over appropriate immuno-adsorbents to remove antibodies cross-reacting with Human
- 抗体名:
Goat anti-Mouse IgG2a (subclass specific), HRP conjugated
- 规格:
1 ml
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum
: Total mouse IgG Ig Type: mouse IgG specific goat IgG Applications: Western blot ELISA Preparation Produced in goats immunized with purified total mouse IgG. Mouse IgG1, mouse IgG2A, mouse IgG2B and mouse IgG3 specific IgG was purified by mouse IgG
T-cell dependent antigen specific in situ staining (Pe)
HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’. Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide
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