- 询价
- SF006
- 2025年07月14日
- Stem Cell Culture
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 应用范围：
Stem Cell Culture
- 宿主：
0
- 抗原来源：
0
- 库存：
大量
- 是否单克隆：
0
- 规格：
100 mL
1. Thaw Accutase™ to room temperature.
2. Wash plate, flask or beads with sterile PBS.
3. Add Accutase™ to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.
4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).
5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.
- Mouse Embryonic Stem Cells
- Induced Pluripotent Stem Cells
1X Accutase™ enzymes in Dulbecco's PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
详细描述见链接：http://www.millipore.com/catalogue/item/SF006
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文献和实验Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)
实验试剂 1. Absolute ethanol (96%-100%) 实验设备 1. Nuclease-free 50 ml centrifuge tube 2. Water bath, incubator or heating block preset at 65°C 3. Magnetic separation device for 50 ml tube
space and out the other end of the bone into a 1.5 mL tube. Plate 100 µL onto positively charged glass slides with a cytospin centrifuge and stain with Wright-Giemsa. This will preserve the cellular morphology and will allow an accurate differential cell
of plasmids, we have used everything from CsCl-banded DNA , quick prep DNA , and heat denatured, transformed bacteria. In the latter case, a single bacterial colony (or as small a portion of a frozen stock as possible) was added to 0.5 ml of TE, vortexed
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