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- RW1914250
- 2025年07月10日
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- Mixed Cellulose Esters
- Cellulose Web
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RW PrefiltersRW prefilters can be used to remove contaminants from a variety of liquids and gases. The filters have a high dirt holding capacity and a low pressure drop, which makes them ideally suited for reducing contaminant levels in advance of sterilizing-grade filters. RW filters are made from non-shedding materials. The filters retain contamination on the surface of the cellulose membrane. |
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详细描述见链接：http://www.millipore.com/catalogue/item/RW1914250
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文献和实验20‐ml Luer‐lock sterile disposable plastic syringes 0.2‐µm membrane sterile syringe filters (25‐mm diameter)
Myosin Light Chain Preparation
Buffer A.Homogenizing Buffer: 28 liters: 50 mM KCl,20 mM Tris (pH 8.0),15 mM MCE, 0.2 mM MgAc2 (350 ml 4 M KCl,280 ml 2 M Tris,28 ml 15 M MCE,1.20 g MgAc2) Buffer B.Triton X-100 Buffer: 4 liters of Buffer A plus 40 ml X-100 (1% Triton final) Buffer C
) 2 x binding buffer: 1 M NaCl; 20 mM Tris pH 7.5; 2 mM EDTA; 0.1 % SDS [50 ml : 12.5 ml 4 M NaCl; 1 ml 1 M Tris; 200 µl 0.5 M EDTA; 500 µl 10 % SDS] 1 x binding buffer: 0.5 M NaCl; 10 mM Tris pH 7.5; 1 mM EDTA; 0.05 % SDS [50 ml: 6.25 ml
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