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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Radioimmunoassay
- 宿主:
0
- 适应物种:
Rat
- 抗原来源:
0
- 库存:
大量
- 是否单克隆:
0
- 规格:
250 tubes
- Endocrine Kits
- Radioimmunoassays (RIA)
- Obesity
- Metabolic Disorders
- Inter-assay: 8.5–9.4
- Intra-assay: 1.4–4.6
- Radioactive
- 125 I
ED50 = 0.8 ± 0.04 ng/mL
ED20 = 3.0 ± 0.36 ng/mL
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/RI-13K
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文献和实验). Leave tubes on ice and repeat Steps 13�15 for the next 6�12 plates of cells. When all cells are collected, centrifuge all tubes at 250g for 5 min at 4 °C. Discard the supernatant and resuspend the cell pellet in 10 ml of ice
分离培养大鼠或小鼠小脑神经元 Dissociated Cultures of Cerebellar Neurons
also use Sprague-Dawley and Wistar rats yield of cells is approx 1.5 X 107 cells per P8 rat original protocols call for fetal bovine serum; however, calf serum was used and seems satisfactory insulin is used for survival, rather than IGF-1 of ref
, Hedgehog, and insulin pathways, as well as the activationof nuclear factor (NF)-kB-mediated gene transcription. These signaling pathways regulate gene transcription,cell cycle, apoptosis, inflammation, glucose metabolism, stem-cell renewal
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