仅供体外研究使用,不用于临床诊断!
Mix 5 ml
CST 8899S LC3B (D11) XP® Rabbit mAb (PE Conjugate) 100 ul
CST 89001S SimpleChIP® Human ID1 5'UTR Primers 500 ul
CST 8900SC Human Interleukin-1β (hIL-1β) 10 ug
CST 8900SF Human Interleukin-1β (hIL-1β) 10 ug
CST 8902SC Human Tumor Necrosis Factor-α (hTNF-α) 10 ug
CST 8902SF Human Tumor Necrosis Factor-α (hTNF-α) 10 ug
CST 8904SC Human Interleukin-6 (hIL-6) 10 ug
CST 8904SF Human Interleukin-6 (hIL-6) 10 ug
CST 89052S SimpleChIP® Mouse TULP4 Promoter Primers 500 ul
CST 8905SC Human Interleukin-13 (hIL-13) 5 ug
CST 8905SF Human Interleukin-13 (hIL-13) 5 ug
CST 8907SC Human Interleukin-2 (hIL-2) 10 ug
CST 8907SF Human Interleukin-2 (hIL-2) 10 ug
CST 8908SC Human Vascular Endothelial Growth Factor-121 (hVEGF<sub>121</sub> ) 10 ug
CST 8908SF Human Vascular Endothelial Growth Factor-121 (hVEGF<sub>121</sub> ) 10 ug
CST 8911SC Human Leukemia Inhibitory Factor (hLIF) 10 ug
CST 8911SF Human Leukemia Inhibitory Factor (hLIF) 10 ug
CST 8912SC Human Platelet-Derived Growth Factor BB (hPDGF-BB) 10 ug
CST 8912SF Human Platelet-Derived Growth Factor BB (hPDGF-BB) 10 ug
CST 8913SC Human Platelet-Derived Growth Factor AA (hPDGF-AA) 10 ug
CST 8913SF Human Platelet-Derived Growth Factor AA (hPDGF-AA) 10 ug
CST 8915LC Human Transforming Growth Factor β1 (hTGF-β1) 10 ug
CST 8915LF Human Transforming Growth Factor
样本处理及要求:
1. 血清:将收集于血清分离管的全血标本在室温放置2小时或4℃过夜,然后1000×g离心20 分钟,取上清即可,或将上清置于-20℃或-80℃保存,但应避免反复冻融。
2. 血浆:用EDTA或肝素作为抗凝剂采集标本,并将标本在采集后的30分钟内于2-8℃ 1000×g离心15分钟,取上清即可检测,或将上清置于-20℃或-80℃保存,但应避免反复冻融。
3. 组织匀浆:用预冷的PBS (0.01M, pH=7.4)冲洗组织,去除残留血液(匀浆中裂解的红细胞会影响测量结果),称重后将组织剪碎。将剪碎的组织与对应体积的PBS(一般按1:9的重量体积比,比如1g的组织样品对应9mL的PBS,具体体积可根据实验需要适当调整,并做好记录。推荐在PBS中加入蛋白酶抑制剂)加入玻璃匀浆器中,于冰上充分研磨。为了进一步裂解组织细胞,可以对匀浆液进行超声破碎,或反复冻融。最后将匀浆液于5000×g离心5~10分钟,取上清检测。
4. 细胞培养物上清或其它生物标本:请1000×g离心20分钟,取上清即可检测,或将上清置于-20℃或-80℃保存,但应避免反复冻融。
注:标本溶血会影响最后检测结果,因此溶血标本不宜进行此项检测。