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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Laboratory Filtration
- 宿主:
0
- 抗原来源:
0
- 库存:
大量
- 是否单克隆:
0
- 规格:
25
Product Images
- Mouse Embryonic Stem Cells
- Human Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Cells
- Pancreatic Stem Cells
- Cardiac Stem Cells
- Induced Pluripotent Stem Cells
Product Family Information
EcoStand™ Tube HolderThe new EcoStand tube holder is an environmentally responsible alternative to conventional petroleum-based plastics. In addition to being made from a biodegradable corn-based resin, the EcoStand also features a user-friendly design for both the 50 mL and 15 mL tube formats. |
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Steriflip® Filter UnitsThe Steriflip filter unit is designed to work with standard 50 mL centrifuge tubes, which eliminates sample transfer. To filter, just attach the unit to a 50 mL centrifuge tube containing your sample, flip it over, and apply the vacuum. When finished, the filtrate collects in the attached 50 mL centrifuge tube. |
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/SE1M179M6
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文献和实验Transgenic Mouse Core Facility
-I two more times. Add an equal volume CHCl3, vortex, spin. Remove aq. to Millipore ultrafree MC filter units (0.22um) will need several. (#UFC30GV25 - pk. 25 - 0.5mL) Microfuge at speed 7, 1 min. at RTo. Remove the basket portion from the tubes. 2.Ethanol
减小,膜对低分子量蛋白的结合就越牢固。 通常用 0.45μ m 和 0.2μm 两种规格的 NC 膜。 大于20kD 的蛋白可用 0.45μm 的膜, 小于 20kD的蛋白就要用 0.2μ m 的膜了, 如用0.45μ m 的膜就会发生“Blowthrough”的现象。PVDF 膜灵敏度、分辨率和蛋白亲和力比常规的膜要高,非常适合于低 分子量蛋白的检测。但 PVDF 膜在使用之前必需用纯甲醇浸泡饱和 1-5 秒钟。 蛋白质常用的转移方法主要有两种:槽式湿转和半干转移。前者操作容易
转膜设备:湿转 Bio-Rad 蛋白来源:大鼠血管平滑肌细胞 蛋白名称(可保密):保密 蛋白分子量:72 KD,42 KD 和22 KD WB用膜类型、孔径:一般的PVDF膜 转膜方式(恒压、恒流):湿转,恒流一张膜0.26 A,两张膜0.3 A。 转膜
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