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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Immunohistochemistry
- 宿主:
Mouse
- 库存:
大量
- 适应物种:
Bovine;Chicken;Human
- 抗原来源:
0
- 是否单克隆:
1
- 规格:
100 μg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, H, M, R | IH | Mouse | AlexaFluor®488 | Monoclonal Antibody |
MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (2,3). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (4,5).
SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).
Optimal working dilutions must be determined by end user.
APPLICATION NOTES:
Immunohistochemistry: Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.
- Bovine
- Chicken
- Human
- Mouse
- Rat
- MAP2
- MAP2B
- MAP-2
- MAP2C
- DKFZp686I2148
- MAP2A
- Neuronal & Glial Markers
- Neurofilament & Neuron Metabolism
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
Bovine brain microtubule protein. 详细描述见链接:http://www.millipore.com/catalogue/item/MAB3418X
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文献和实验Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr(图)
secondary or direct FITC-conjugated anti-cyclin antibodies can also be used, the low autofluorescence background seen with 630 nm laser excitation makes Cy5 detection of cyclin expression particularly sensitive. Using dual laser excitation also eliminates
together to calculate compensation in multicolor immunophenotyping experiments that incorporate a LIVE/DEAD® fixable dye by following the protocol outlined below: 1) Gently vortex the ArC™ Amine Reactive Beads Kit and the AbC™ Anti-Mouse Bead Kit
. 2. Combining AbC™ anti-Rat/Hamster and ArC™ Amine Reactive Compensation Bead Kits The ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) is designed to facilitate compensation when using any of the LIVE/DEAD® fixable dead cell stains
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