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Anti-MAP2, AlexaFluor® 488 con

jugated; 100 μg
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  • 询价
  • MAB3418X
  • 2025年07月13日
  • Immunohistochemistry
  • Mouse
  • Bovine;Chicken;Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      Immunohistochemistry

    • 宿主

      Mouse

    • 库存

      大量

    • 适应物种

      Bovine;Chicken;Human

    • 抗原来源

      0

    • 是否单克隆

      1

    • 规格

      100 μg

    Species Reactivity Key Applications Host Format Antibody Type
    B, Ch, H, M, R IH Mouse AlexaFluor®488 Monoclonal Antibody
    Description:
    Anti-MAP2, Alexa Fluor® 488 conjugated
    Clone:
    AP20
    Specificity:
    Recognizes MAP-2 (microtubule associated protein-2). MAP-2 is one of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (1).

    MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (2,3). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (4,5).

    SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).
    Key Applications:
    Immunohistochemistry
    Application Notes:
    Immunohistochemistry

    Optimal working dilutions must be determined by end user.

    APPLICATION NOTES:

    Immunohistochemistry: Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.
    Species Reactivity:
    • Bovine
    • Chicken
    • Human
    • Mouse
    • Rat
    Isotype:
    IgG1
    Immunogen:
    Bovine brain microtubule protein.
    Control:
    Brain, Neuronal culture, Human glioblastoma T98G cells
    Presentation:
    Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.
    Storage Conditions:
    Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    Gene Symbol:
    • MAP2
    • MAP2B
    • MAP-2
    • MAP2C
    • DKFZp686I2148
    • MAP2A
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    • Neuronal & Glial Markers
    • Neurofilament & Neuron Metabolism
    Usage Statement:
    • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    AlexaFluor®488
    Host:
    Mouse
    Concentration:
    1 mg/mL
    Antibody Type:
    Monoclonal Antibody

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    Bovine brain microtubule protein. 详细描述见链接:http://www.millipore.com/catalogue/item/MAB3418X

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    图标文献和实验
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    • AbC Anti-Mouse Bead Kit

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    • AbC Anti-Rat/Hamster Bead Kit

      . 2. Combining AbC™ anti-Rat/Hamster and ArC™ Amine Reactive Compensation Bead Kits The ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) is designed to facilitate compensation when using any of the LIVE/DEAD® fixable dead cell stains

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