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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
ELISA;Flow Cytometry
- 宿主:
Mouse
- 适应物种:
Mammals
- 库存:
大量
- 抗原来源:
0
- 是否单克隆:
1
- 规格:
100 μg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ma | ELISA, FC, IC, IH, WB | Mouse | Purified | Monoclonal Antibody |
- ELISA
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
Immunocytochemistry: 1:100 - 1:500 for assessment of intracellular GSH distribution.
ELISA: 1:100,000 - 1:200,000 to quantify GSH at >2 mM in biological specimens.
Flow cytometry: 1:100 - 1:1,000
Cells were fixed with cold 1% PFA for 5 min and vortexed into methanol (-20°C) containing 5mM NEM (10 min on dry ice) or 10 μM NEM (N-ethyl maleimide) for 30 min at RT.
Optimal working dilutions must be determined by end user.
Positive Control: Peripheral blood mononuclear cells (PBMC) treated with NEM
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
GS-NEM conjugated to KLH 详细描述见链接:http://www.millipore.com/catalogue/item/MAB3194
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文献和实验Methods to Detect Protein Glutathionylation
for protein of interest 50 mM NaH 2 PO 4 , pH 7.4 ( appendix 2A ) 100 mM GSSG (oxidized L‐glutathione) in H 2 O
Glutathione- a microassay for determining glutathione content in cells 检测细胞谷胱苷肽含量 Laboratory of P.J.Hansen,Dept.of Animal Sciences,University of Florida http://www.animal.ufl.edu/hansen/protocols/gsh.htm The following assay is modified
107 的 PBMC 细胞用 100μl 的 Buffer 稀释(Buffer CI:含 0.5% BSA 的 PBS 缓冲液,1 mM EDTA) · 加入多种生物素化抗体,室温下孵育 5 分钟。生物素化的抗体包含以下几种,每种抗体 0.5 μg:CD235a (erythrocytes), CD14 (monocytes), CD3 (T cells), CD15 (granulocytes), CD19 (B cells), CD36 (thrombocytes), CD123
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