产品封面图

Anti-Glutathione: N-ethylmalei

mide adduct, clone 8.1GSH; 100 μg
收藏
  • 询价
  • MAB3194
  • 2025年06月28日
  • ELISA;Flow Cytometry
  • Mouse
  • Mammals
avatar
11金牌会员
  • 企业认证

    点击 QQ 联系

    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      ELISA;Flow Cytometry

    • 宿主

      Mouse

    • 适应物种

      Mammals

    • 库存

      大量

    • 抗原来源

      0

    • 是否单克隆

      1

    • 规格

      100 μg

    Species Reactivity Key Applications Host Format Antibody Type
    Ma ELISA, FC, IC, IH, WB Mouse Purified Monoclonal Antibody
    Description:
    Anti-Glutathione: N-ethylmaleimide adduct, clone 8.1GSH
    Clone:
    8.1GSH
    Specificity:
    Reactive with thiol-modified glutatione. Does not react with oxidized glutathione (GSSG) or glutathione (GSH) in mixed disulfide linkage with proteins. MAB3194 is specific to the glutathione:N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide. For use in ELISA or flow cytometry, sample must first be reacted with NEM.
    Key Applications:
    • ELISA
    • Flow Cytometry
    • Immunocytochemistry
    • Immunohistochemistry
    • Western Blotting
    Application Notes:
    Immunohistochemistry: 1:100 (paraformaldehyde fixation)

    Immunocytochemistry: 1:100 - 1:500 for assessment of intracellular GSH distribution.

    ELISA: 1:100,000 - 1:200,000 to quantify GSH at >2 mM in biological specimens.

    Flow cytometry: 1:100 - 1:1,000

    Cells were fixed with cold 1% PFA for 5 min and vortexed into methanol (-20°C) containing 5mM NEM (10 min on dry ice) or 10 μM NEM (N-ethyl maleimide) for 30 min at RT.

    Optimal working dilutions must be determined by end user.

    Positive Control: Peripheral blood mononuclear cells (PBMC) treated with NEM
    Species Reactivity:
    Mammals
    Isotype:
    IgG1
    Immunogen:
    GS-NEM conjugated to KLH
    Presentation:
    Liquid in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
    Storage Conditions:
    Maintain at 2-8°C in undiluted.
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    Oxidative Stress
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    Purified
    Host:
    Mouse
    Concentration:
    1 mg/mL
    Antibody Type:
    Monoclonal Antibody

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    GS-NEM conjugated to KLH 详细描述见链接:http://www.millipore.com/catalogue/item/MAB3194

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验
    • Methods to Detect Protein Glutathionylation

      for protein of interest 50 mM NaH 2 PO 4 , pH 7.4 ( appendix 2A ) 100 mM GSSG (oxidized L‐glutathione) in H 2 O

    • 检测细胞谷胱苷肽含量

      Glutathione- a microassay for determining glutathione content in cells 检测细胞谷胱苷肽含量 Laboratory of P.J.Hansen,Dept.of Animal Sciences,University of Florida http://www.animal.ufl.edu/hansen/protocols/gsh.htm The following assay is modified

    • 这种阴选方法快速简单,10 分钟得到想要的活性细胞!

      107 的 PBMC 细胞用 100μl 的 Buffer 稀释(Buffer CI:含 0.5% BSA 的 PBS 缓冲液,1 mM EDTA) · 加入多种生物素化抗体,室温下孵育 5 分钟。生物素化的抗体包含以下几种,每种抗体 0.5 μg:CD235a (erythrocytes), CD14 (monocytes), CD3 (T cells), CD15 (granulocytes), CD19 (B cells), CD36 (thrombocytes), CD123

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    询价
    默克生命科学
    2025年07月15日询价
    询价
    北京博蕾德生物科技有限公司
    2025年07月08日询价
    ¥693.10
    北京智杰方远科技有限公司
    2025年09月12日询价
    询价
    安元生物科技(南京)有限公司
    2025年03月31日询价
    询价
    Agrisera AB
    2025年05月14日询价
    Anti-Glutathione: N-ethylmaleimide adduct, clone 8.1GSH; 100 μg
    询价