EmbryoMax® ES Cell Qualified 2
X Cell Culture Freezing Medium, Formulated with 20% DMSO & Fetal- 询价
- ES-002-D
- 2025年07月15日
- Stem Cell Culture
- 0
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Stem Cell Culture
- 宿主：
0
- 库存：
大量
- 抗原来源：
0
- 是否单克隆：
0
- 规格：
50 mL
The sera utilized in this medium is approved by the United States Department of Agriculture (USDA) to ensure that sera is free of diseases such as Bovine Spongiform Encephalopathy (BSE).
Wash Plate with PBS. Pick colonies into 96 wll plate (U shaped wells) with 35 µl of trypsin / well. Pick roughly 48 colonies, incubate at 37 °C for 10 minutes. Use Multichannel pipet to break up colonies. Transfer directly into 96 well plate with feeders and 150 µl of ES medium (without selection drug). Feed every day with ES medium containing selection drug (150 µg /ml neomycin, or 50 µg /ml of hygromycin). After 2 days cells should be ready to be frozen. Wash with PBS add 35 µl of Trypsin, incubate at 37 °C for 10 minutes, add 65 µl of ES medium. Use multichannel pipet to disperse cells. Transfer 65 µl into replica plate (U shaped wells) containing 65 µl of cold 2 x ES freezing medium. Wrap with parafilm, place on dry ice for 20 minutes, and transfer to -80 °C.
- Mouse Embryonic Stem Cells
- Induced Pluripotent Stem Cells
- Maintenance & Expansion
- General Cell Culture
- Cell Culture Supplements
- Stem Cell Reagents
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
详细描述见链接：http://www.millipore.com/catalogue/item/ES-002-D
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验ES and TS cell freezing/thawing
Needed: ES cell freezing medium (2x) 2x ES cell freezing medium should be made up fresh each time it is to be used, and should comprise freshly prepared 60% DMEM+, 20% FCS, and 20% DMSO (Sigma, Cat. No.D-5879). Freezing
ES Cell Culture and Manipulation
culture dishes containing nearly confluent ES cell cultures Phosphate-buffered saline (PBS) (incomplete) Lysis buffer (150 mM NaCl, 2 mM EDTA, 1% SDS, 20 mM Tris-HCl [pH 8.0], µg/ml proteinase K)
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
to remove any debris. The embryos are washed in KSOM medium and cultured in organ culture dish in KSOM at 37o C, 5% CO2. Production of tetraploid embryos Fusion of the blastomeres of 2-cell stage embryos occurs when a square pulse is applied
技术资料暂无技术资料 索取技术资料
