SBI,pPACKH1 HIV Lentivector Pa

pPACK Packaging Plasmid Mix是一种优化的包装质粒混合物，其量足够10-cm的组织培养板（或75cm2瓶）里慢病毒载体表达构建的10次转染。阳性质控表达构建使用copGFP报告基因和shRNA序列定靶于萤火虫荧光素酶，提供量足够6个质控共转染pPACK packaging plasmid mix。 
    pPACKH1™ Lentivector Packaging Kit——包装基于HIV的慢病毒表达载体构建
    200ul pPACKH1 Packaging Plasmid Mix：pPACKH1-GAG、pPACKH1-REV和pVSV-G质粒的混合物（0.5ug/ul）

Generate high titers of transduction-ready pseudoviral particles with this HIV-based Lentivector Packaging Kit

• Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
• Easily generate high-titer lentiviral preparations
• Efficiently deliver most third-generation lentivector expression constructs
• Successfully reach:
  A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles
  A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more
• A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles

Overview

Produce high-titer lentivirus with this HIV-based Lentivector Packaging Kit

Designed to efficiently package most third-generation lentivectors, the pPACKH1 HIV Lentivector Packaging Kit makes it easy to deliver and express lentiviral constructs in a wide-range of mammalian cells.

• Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
• Easily generate high-titer lentiviral preparations
• Efficiently deliver most third-generation lentivector expression constructs
• Successfully reach:
  • A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles
  • A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more

The pPACKH1 Packaging Plasmid Mix consists of three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudotyped lentiviral particles. Just co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs, etc.

 

How It Works

The lentivirus packaging, production, and transduction workflow

Supporting Data

SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism