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文献和实验Large Scale Tubulin Preparation
3.5 liters of 10X CB for 10-12 brain prep. GTP : Sigma Type IIS- # G-8752 ATP : Sigma Grade 1- # A-2383 Glycerol : 2-3L prewarmed to 37¡C (store overnight in 37¡C incubator) III. Pouring a 1L Phosphocellulose (PC) Column
Large Scale Tubulin Preparation
Back to protocols I. Tubulin Prep Outline Pre-Prep : 1. Call slaughterhouse and request fresh brains to be picked up the morning of prep 2. Pour and equilibrate phosphocellulose column 3. Make buffers 4. Ensure
Large Scale Tubulin Preparation
Back to protocols I. Tubulin Prep Outline Pre-Prep : 1. Call slaughterhouse and request fresh brains to be picked up the morning of prep 2. Pour and equilibrate phosphocellulose column 3. Make buffers 4. Ensure
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