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文献和实验µl PlateBlock™ for 2 h 4. Aspirate or tap the plate (do not wash) 5. Stabilization with Liquid Plate Sealer® animal-free for 2 - 15 min 6. Aspirate or tap the plate (do not wash), dry and store 7. Dilute the patient sample (1:100) in LowCross
An Economic PCR Enhancer for GC-Rich PCR Templates
several different polymerases The 5x concentrated Combinatorial Enhancer Solution (CES) contains: 2.7 M betaine 6.7 mM DTT 6.7% DMSO and 55 μg/ml BSA Add this solution to your PCR and see if it works Some hints if your PCR
Detection of Reactive Oxygen Intermediate Production by Macrophages
the times of addition for PMA (200 n M ), deoxycholate/Tween (DOC), NADPH (0.6 m M ), and SOD (300 U/mL). (Reproduced with permission from ref. 5 ). Fig. 2. H 2 O 2 release
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