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文献和实验Flow Cytometry of Bacterial Membrane Potential and Permeability
This chapter describes reliable flow cytometric methods for assessment of two important physiologic characteristics of bacteria, membrane potential and membrane permeability, which can provide indications of the effects of antimicrobial
MitoProbe DiIC1 Mitochondrial Membrane Potential Protocol
(V13241, prepared according to instructions accompanying that kit). 5) Incubate the samples at 37°C for 15 minutes. (37°C is important to maintain membrane potential.) 6) Add 400 μL annexin binding buffer. 7) Analyze
Comparing Bacterial Membrane Interactions of Antimicrobial Peptides and Their Mimics
to measure the membrane activities of AMPs and antimicrobial peptidomimetics: (1) a calcein leakage assay to investigate interactions between AMPs/peptidomimetics with large unilamellar vesicles and (2) a potential-sensitive dye-based depolarization assay
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