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文献和实验Embryo Dissection and Micromanipulation Tools
, such as the Dumont 5 or 5A (Fine Science Tools), are adequate for membrane removal and most micromanipulations. They do not rust easily, but they cannot be sharpened to as fine a point as carbon-steel forceps. To sharpen the forcep points, use a fine stone
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
pseudopregnant females; Instruments: Scissors; Semken forceps (straight or curved with serrated tips); Forceps with 1 x 2 teeth; Dumont ss/mc or No. 5 forceps; Serrefine (e.g. Fine Scientific Tools: 1 8050-28 or 8051-28);
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
, sterilized (#5 pointed) (Fine Science Tools 11252-30) Hemacytometer Incubator preset to 37°C (humidified, 5% CO2 ) Knife, microsurgical (5-mm) (MSP/Surgical Specialties 7516) Parafilm Pipette (P200; P20 may be used in place of P200
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