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文献和实验Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles
., by sonication of membrane proteins with lipids), detergent-based methods are more widely used and appear to be more reproducible (1 ). If a particular protein is to be purified in an active form, then particular attention must be paid to the choice of detergent
Preparation of active proteins from inclusion bodies.
Background: It is often difficult to obtain soluble and active proteins from expression in prokaryotes. Often, overexpression leads to the production of inclusion bodies :insoluble aggregates of misfolded protein
Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae
cells as an intermediate stage. We suggest that small-to mid-scale recombinant protein production (mg-g level) can be achieved in T. ni larvae with relative ease.
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