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文献和实验pellets by centrifuging at 3,500 xg for 20 minutes. Resuspend the nuclear pellet in 80 ml of cold 0.25 Sucrose containing 0.0033 M Calcium Acetate. 4 Obtain three cellulose nitrate centrifuge tubes and place 25 ml
Protein purification: skeletal muscle myosin
Equipment: • centrifuge with GSA-rotor • cellulose nitrate tubes • gauze • DEAE-sephadex column (2.5 x 60 cm) Chemicals: KCl, EDTA, MgCl2, K/PO4, ATP, DTT Have ready: solution A: 0.3 M KCl, 0.15 M potassium
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELS
123.66 g boric acid 14.89 g EDTA Adjust pH to 8.3. QS to 2 liters. Autoclave. (Biotechniques 10:182, 1991 claims that filtering up to a 20x TBE solution through 0.2 - 0.45µ cellulose acetate or cellulose nitrate filters prevents formation
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