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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
off the PBS. Add 100 µL of 1X PBS to each tube. Add 4 µg of antibody to one tube and 4 µg of preimmune serum to the other. Rotate for 4-6 h at 4°C. 31. Using the magnetic stand, aspirate the supernatants and wash the beads in each tube
Size‐Exclusion Chromatography with On‐Line Light Scattering
onto a Superdex 75 column (Pharmacia) with PBS as eluent at a flow rate of 0.5 ml/min. (A ) 1 mg/ml of native RNase; (B ) 1 mg/ml of RCM‐RNase; (C ) chromatograms in panels A and B are put onto the same scale for comparing the LS/RI ratios of native and reduced
RNA-chromatin immunoprecipitations (RNA-ChIP) in mammalian cells
(including an equal amount of a normal IgG control) and immune complexes were allowed to form by slow mixing on a rotating platform at 4ºC overnight. Although antibody concentrations will vary, a guideline can be to begin within the range of 0.5�5µg/ml
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