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Vybrant® DyeCycle Green and Orange Stains

, buffer, and experimental condition. For a given experiment, each flow cytometry sample should contain the same number of cells, as sample-to-sample variation in cell number leads to significant differences in fluorescence signal. If a Vybrant® DyeCycle™

Dynabeads® Co-Immunoprecipitation Kit

Dynabeads® Co-Immunoprecipitation Kit Magnet: e.g. DynaMag™-2  Mixer allowing rotation or tilting of tubes. Antibodies of your choice. 实验步骤           Day 1 1.        Weigh out the appropriate

WOLF®流式细胞分选仪介绍

细胞经过10x Genomics进行建库测序，经Cell Ranger 分析结果显示，未经分选的样品和经 WOLF®分选的样品分别捕获了2256和2038个细胞。WOLF®分选的细胞样本检测到的每个细胞基因中位数（1101）是未分选的样本（456）的两倍以上。此外，WOLF® 分选的细胞样品中检测到总基因数也更多。每个细胞更多的基因中位数和总基因数都表明经过 WOLF® 分选的样品的文库丰富度更高。我们还检测了与细胞无关的 reads 读数。未分选样品中游离的 RNA 污染数量几乎是 WOLF®