QIAGEN凯杰特约代理商|150023 REPLI-g M

PureLink RNA Mini Kit

application. 3.        RNA Storage Store the purified RNA on ice for immediate use. For long–term storage, keep the purified RNA at –80°C. Perform DNase I treatment after purification (refer to the PureLink™ RNA Mini Kit manual) to assure highly

Isolation of Total RNA from Animal Cells use RNeasy Mini Kit

3)   Transfer cells to an RNase-free centrifuge tube and pellet by centrifugation at    300 x g for 5 min. (In our lab, use 1800rpm in the eppendorf centrifuge 5415D). Cell pellets can be stored at –70°C for later use or used directly in the procedure

E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol

again. Centrifuge at maxi speed (13,000 x g) for 3 minutes at room temperature (22-25℃). The ETR Reagent should form a blue layer at bottom of the tube. 7. Transfer the top aqueous phase (cleared lysate) into a new 2 ml microtube and add 0.5 volume of absolute