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Isotype-specific immunostaining with mouse primary antibodies on mouse tissue

) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a

Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas

me why this works!) before cloning out for variants. b) You can screen by ELISA or with mAb coupled RBC using mouse monoclonal antibodies against the Y3 derived Kappa 1a (RG11/15.5) and the specific DA Kappa 1b (G9/1.3), or the appropriate heavy chain (eg NORIG

【交流】谁用过RD公司的HRP酶标羊抗鼠IgG试剂盒.

ORDERING INFORMATION Catalog Number: HAF007 Lot Number: FIM06 Size: 1 mL Formulation: 0.2 μm filtered solution in PBS with stabilizers Storage: 2 - 8° C Specificity: mouse IgG1, mouse IgG2A, mouse IgG2B, mouse IgG3 heavy and light chains Immunogen