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Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas

7.16 anti-rat IgG2b). -------------------------------------------------------------------------------- 5) For full details see: Clark, M.R., & Waldmann, H. Production of murine monoclonal antibodies in Methods in Hematology 13:1-20 (1986

【交流】谁用过RD公司的HRP酶标羊抗鼠IgG试剂盒.

ORDERING INFORMATION Catalog Number: HAF007 Lot Number: FIM06 Size: 1 mL Formulation: 0.2 μm filtered solution in PBS with stabilizers Storage: 2 - 8° C Specificity: mouse IgG1, mouse IgG2A, mouse IgG2B, mouse IgG3 heavy and light chains Immunogen

Double Immunohistochemistry.

by staining in simple IHC the relevant heterologous primary antibodies. E.g. many goat anti mouse IgG1 heavy chain do stain rat IgG2a and IgG2b .     Procedure:  First stain  HRP Developing solution  Second stain  AP