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LINEAGE ANALYSIS USING RETROVIRAL VECTORS

immediately before use by diluting 1:50 into 1 x PBS. Paraformaldehyde Fixative (4%):4 g solid paraformaldehyde2 mM MgC121.25 mM EGTA (0.25 ml of a .5M EGTA stock pH 8.0) in 100 ml PBS, pH 7.2-7.4Heat H20 to 60°C. Add paraformaldehyde. Add NaOH

（共享）免疫组织化学染色FAQ

' In contrast, aldehydes combine mainly with protein nitrogen atoms. Cross-links between the lysine side-chain amino group and the amide nitrogens of peptide linkages are thought to do most of the fixing. Various carbodiimides have been used as fixatives