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文献和实验Bacterial Mediated Gastrointestinal Inflammation
Campylobacter jejuni strain 81-176 or the commensal Escherichia coli strain NC 101.
from Stiles (1983) Methods in Enzymology 101: 296-297) Grow 3ml culture of yeast strain carrying your YAC of interest in selective media (e.g. CM URA- TRP-) @ 30 C for 1-2 overnights. Add culture to 50ml YPD media in flask, shake O/N 30 C.
Hemp (Cannabis sativa L.) suspension culture cells were transformed with Agrobacterium tumefaciens strain EHA101 carrying the binary plasmid pNOV3635. The plasmid contains a phosphomannose isomerase (PMI) selectable marker gene. Cells
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