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文献和实验Measurement of Cation Movement in Primary Cultures Using Fluorescent Dyes
1 mM Ca2+ ionophore (ionomycin or 4Br‐A23187; Molecular Probes) in dimethyl sulfoxide (DMSO) 100 mM CaCl 2 in H 2 O
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
: [active] 1mM, stock 100mM, MW 469, stock = Sigma B1381 47mg/ml H2O, dilute 100x Ca ionophore: [active] 5mM, stock 5mM, MW 524, stock = Sigma 7272 2.6mg/ml DMSO, dilute 1000x PMA: [active] 10mM, stock 10mM, MW 616, stock = 6.1mg/ml DMSO, dilute 1000
ETC ** Carbodiimides as fixatives Question. Does anyone know what carbodiimide is and how it works? Answer. The name "carbodiimide" is sometimes applied to cyanamide (hydrogen cyanamide, H2NCN. Don't confuse this with calcium cyanamide, CaNCN
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