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文献和实验Calpains in the Lens and Cataractogenesis
, buthionine sulfoximine, calcium ionophore A23187, hydrogen peroxide, diamide, xylose, galactose, and streptozotocin; and in several animal models, Nakano mice, UPL hereditary rat cataract, Shumiya cataract rat (SCR), and transgenic
Measurement of Cation Movement in Primary Cultures Using Fluorescent Dyes
1 mM Ca2+ ionophore (ionomycin or 4Br‐A23187; Molecular Probes) in dimethyl sulfoxide (DMSO) 100 mM CaCl 2 in H 2 O
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
: [active] 1mM, stock 100mM, MW 469, stock = Sigma B1381 47mg/ml H2O, dilute 100x Ca ionophore: [active] 5mM, stock 5mM, MW 524, stock = Sigma 7272 2.6mg/ml DMSO, dilute 1000x PMA: [active] 10mM, stock 10mM, MW 616, stock = 6.1mg/ml DMSO, dilute 1000
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