invitrogen特约代理商|16500500 Ultra

Agarose Gel Electrophoresis

and applied voltage. Invitrogen offers a range of UltraPure™ agarose products to meet your nucleic acid electrophoresis needs. As with all Invitrogen UltraPure™ reagents, these products are made from the highest purity biochemicals for maximum reliability

Multiple Tissue Northern (MTN™)Blots

。 2.mRNA的变性胶电泳验证。以确保得到的是纯净、未受破坏的mRNA,并且保证Genomic DNA的含量小于1%。（尽管Total RNA可以用来mRNA代替制作MTN™膜，Clontech公司的膜仍然mRNA,使灵敏度提高约50倍，并大降低非特异选民性背景。实验重复性非常好，您可信赖。） 3.变性胶甲醛/1.2%琼脂糖电泳分离mRNA。（每个泳道含大约2μg的组织特异性mRNA,并且保证每个泳道可见β-action信号） 4.转于带正电的尼龙膜. 多重的实验信息 1.由于内参RNA分子

Denaturing Agarose Gel Electrophoresis of RNA

as well). Figure 1. Intact vs. Degraded RNA. Two µg of degraded total RNA and intact total RNA were run beside Ambion''s RNA Millennium Markers™ on a 1.5% denaturing agarose gel. The 18S and 28S ribosomal RNA bands are clearly visible in the intact RNA