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文献和实验Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
of 0.1 ml 1.5 M TCA. Centrifuge the reaction mixture at room temperature (10,000 rpm for 5 min) to remove the precipitate. Determine the ammonia released in the supernatant colorimetrically (A480 ) by adding 0.25 ml Nessler reagent into tubes
How to Make Simple Solutions and Dilutions
of a bacterial culture (see figure below) in the process of quantifying the number of viable bacteria in a culture (see figure below). Each step in this example uses a 1 ml total volume. The initial step combines 1 unit volume of bacterial culture (10 ul) with 99
RNA Isolation Protocol (Revised 5-15-2003) Stabilize RNA Start with 15 ml E. coli Culture containing 7.5* 109 cells (OD600 = 0.2 Dilute cells or scale up) Pipet 30 ml of RNAProtect Bacteria Reagent
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