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Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE

to anaphylaxis. Here, we describe the history behind the IgE discovery and the protocol for purifying IgE and anti-IgE in the mouse. This is followed by our ELISA protocol for mouse IgE detection.

secondary antibody review -- data from 99 publications

Signaling 18   IgG   Alexa Fluor 488 immunocytochemistry 1:2000     18   IgG   Alexa Fluor 568 immunocytochemistry 1:2000     19 donkey IgG   HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat

Protocol for anti-HA antibody Western Blotting

seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS