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上海玉博生物科技有限公司
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文献和实验Culture undifferentiated or differentiating ES cells on gelatin-coated coverslips for at least 24-48 hours; Wash once in freshly prepared 1X PBS; Fix in freshly made, filter-sterile 3% paraformaldehyde/1X PBS for 10 minutes at RT
surface coated with SIF, we used a model immunoassay format shown in Scheme 1. A model antigen, mouse Immunoglobulin G (IgG), was immobilized on the surfaces (SIF-coated surface and glass surface used as a reference), and corresponding specific antibodies
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
) and the slide with the coverslip are then positioned on the holding block and held in place by the suspended block. Tweezers METHOD Preparation of Poly-L-Lysine-Coated Slides 1. Place 100-200 slides
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