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上海玉博生物科技有限公司
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文献和实验Largescale nuclear extract preparation
extract by centrifugation (4℃ 10min)in microfuge. 3.Measure protein and aliquot supernatant (100-500mg/tube)and store it at 70℃
Large scale nuclear extract preparation
Hattoti's protocol adapted to cell culture : Hattori M,Tugores A,Veloz L,Karin M,Brenner D (1990)A simplified method for the preparation of transcrip-tionally active liver nuclear extracts.DNA Cell Biol.Vol.10,P.777-781 I.Homogenize cells
Large scale nuclear extract preparat
Hattoti's protocol adapted to cell culture: Hattori M,Tugores A,Veloz L,Karin M,Brenner D (1990)A simplified method for the preparation of transcrip-tionally active liver nuclear extracts.DNA Cell Biol.Vol.10,P.777–781 I.Homogenize cells or tissue
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