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文献和实验interaction network.This has facilitated discovery of novel pluripotency factors and a better understanding of stem cell pluripotency. Here we describe detailed procedures for an in vivo biotinylation system setup in mouse ES cells, and affinity purification
x stock Tris/ Glycine SDS buffer were from National Diagnostics, (Hull, UK).eNOS (NOS 3) affinity purified rabbit polyclonal antibody was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, Calafornia, USA).Goat Anti-Rabbit horseradish peroxidae
Immunohistochemisty-Fluorescence Protocol
Materials Cytokine-specific Primary Antibodies unlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'' or ''BAF'' series) or selected monoclonal antibodies. Secondary Antibodies
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