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上海玉博生物科技有限公司
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文献和实验Expression and Preparation of Fusion Proteins from Recombinant gt1.1 Phages
-level expression, the relative stability of β-galactosidase fusion proteins, and simple approaches to purify the fusion proteins. After the desired clone is detected and purified, it is often necessary to obtain preparative amounts of recombinant protein
Single-molecule analysis of DNA replication by molecular combing
. Wash 3 x 5 minutes in 2xSSC, 50% formamide. Wash 3 x 5 minutes in 2xSSC. Wash 3 x 5 minutes in PBS/T. Proceed with step 5 of “Immunodetection ” section. Use a mouse anti-DIG antibody (Roche) instead of the anti-DNA antibody
29 (MEM 101A, purified IgG1) was used as reported previously [9–11]. FITC-labeled antibodies to CD80, CD86, CD29, and CD18 were obtained from Immunotech (Marseilles, France).2.2. AnimalsC57BL/6 male mice (6–8 weeks old, 17–21 g) were obtained from DAEHAN
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