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文献和实验RAMPAGE: Promoter Activity Profiling by Paired‐End Sequencing of 5′‐Complete cDNAs
and mapping of promoters for analysis of gene expression (RAMPAGE) is a method that harnesses highly specific sequencing of 5??complete complementary DNAs to identify transcription start sites (TSSs) genome?wide. Although TSS mapping has historically relied
Can anyone recommend a good kit for genome walking? I would like to find out the promoter sequence of a known gene. Is genome walking the way to do it?thanks a million for any suggestions!-kiwi-Kiwi-I use the Universal GenomeWalker Kit from BD
Inducing RNAi with siRNA Cocktails Generated by RNase III
products (21 bp range) can effectively mediate RNAi in both mammalian cells (10) and mouse embryos (11). We demonstrate here that the siRNA cocktails produced by complete digestion by RNase III (~12 to 15 bp) are capable of silencing specific genes
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