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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
solution (a small spoonful of nonfat dry milk in 40 mL PBS) Bovine serum albumin (BSA) 3,3''-diaminobenzidine tetrahydrochloride (DAB; Sigma D5637) Detergent (optional, see Step 2) Drosophila (see Steps 7-9) Entellan (EMD
Preparation and Staining of Frozen Tissue Sections
Preparation and Staining of Frozen Tissue Sections for use with Pharmingen reagents I. Preparation of Frozen Sections for Sectioning Materials neeed: 2-methylbutane (isopentane) Liquid Nitrogen Dry ice
with release from anthers but is not essential), then dab anthers onto surface of a 25-30µL drop of germination media. Drop can be on a depression slide or on a coated slide designed to keep samples separate. Use 1 flower per drop. Invert and incubate overnight
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