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上海玉博生物科技有限公司
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文献和实验, but now aseptically remove 0.1 ml of culture from the 10 tube and place it into a new broth tube, which now becomes a 1/10,000 or 10 dilution. Mix the contents and label as 10. 5. Repeat this procedure twice more to produce respectively a 10 and a 10 dilution
Probiotic Attributes of Lactic Acid Bacteria Isolates
in MRS broth at 30° C without shaking and growth and have to be monitored at 650 nm. Adaptation to low pH: Dilute log-phase LAB cultures 100 fold in fresh MRS broth (10 ml) to make a preculture. Dilute again this preculture 100 fold in 100
Expression L19 using Pichia pastoris
nitrogen base) 4x10-5 biotin 1% methanol Dissolve 10g yeast extract and 20g peptone in 700ml water. Autoclave 20 min on liquid cycle. Cool to room temperature then add the following and mix well: 100 ml 1M potassium phophate buffer pH 6 100
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