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上海玉博生物科技有限公司
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文献和实验to as clostridiopeptidase A which is a protease with a specificity for the X-Gly bond in the sequence Pro-X-Gly-Pro, where X is most frequently a neutral amino acid. Such sequences are often found in collagen, but only rarely in other proteins. While many proteases
RNase and DEPC Treatment: Fact or Laboratory Myth
to document the "facts" taught by mentors and technical manuals. One of these potential myths is the use of DEPC treatment to make solutions RNase-free. At Ambion, we have systematically investigated some of the DEPC fables, and the results are discussed
Hybridisation to RNA in Tissue Sections
to a final concentration of 5mM. The down side of adding cold UTP is that the specific activity of the resultant probe decreases. Transcription reaction: For 1 probe: µl Final Conc. 5 x
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